Cervical collagen and hydroxy-prolin ratios physiologic changes of collagen during first trimester. Perinatal Journal 2014;22(3):SE44-45
- İstanbul Bakırköy Sadi Konuk Eğitim ve Araştırma Hastanesi, Biyokimya Bölümü- İstanbul TR
- İstanbul Kanuni Sultan Süleyman Eğitim ve Araştırma Hastanesi, Kadın Hastalıkları ve Doğum Bölümü- İstanbul TR
- İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Biyokimya Anabilim Dalı- İstanbul TR
Ali Gedikbasi, İstanbul Kanuni Sultan Süleyman Eğitim ve Araştırma Hastanesi, Kadın Hastalıkları ve Doğum Bölümü- İstanbul TR,
Conflicts of Interest
No conflicts declared.
The cervical tissue consists mainly of an extracellular matrix rich in collagen; important fort the biomechanical properties. During normal gestation, the biomechanical strength of the cervix is determined by the collagen concentration. This study demonstrates the differences and changes of cervical collagen in first trimester abortions and its relation with age and parity.
Cervical punch biopsies were obtained from 59 patients divided into two groups: first trimester abortion group (study group; n= 45), and patients with cervical biopsy due to gynecologic reasons (control group; n= 14). According to their gestational weeks, 45 first trimester pregnant women (median age=28.2) were divided into 3 groups (<7 weeks, 7-9 weeks, >9 weeks). Biochemical testing was performed and collagen quantity (as μg OH-prolin/mg dry weight) was determined. Hydroxyproline was measured in cervix tissue by the method of Switzer. Finally, the results of collagen and its relation with age and parity were investigated.
Collagen quantity was lower in the first trimester pregnant women group (13.06 ± 4.17 µg OH-prolin/mg tissue) (p= 0.0001) compared with the control group (24.48±12.87). Moreover, when data were adjusted for gestational weeks, no difference in collagen quantity was found between <7 weeks and control groups (17,01 ± 1,11 µg OH-prolin/mg tissue) (p= 0,047). However the collagen quantities of 7-9 weeks (12.07±3.85 µg OH-prolin/mg tissue) and >9 weeks (11.16±4.39 µg OH-prolin/mg tissue) groups were statistically lower compared with the control group (p=0.0001). Furthermore, the results of first trimester pregnancy group were evaluated for age and parity. Lineer regression analyses shows that collagen increases with age and decreases with parity.
Our study shows that, the collagen content of cervix started to decrease as early as first trimester of gestation. We observed that, from the beginning of 7th gestational week, cervical collagen starts to decrease. In accordance with the literature knowledge, collagen quantity increases with age and with regard to parity.
Cervical collagen, first trimester physiologic changes