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Online ISSN
1305-3124

Established
1993

Editors-in-Chief
​Cihat Şen, ​Nicola Volpe

Editors
Daniel Rolnik, Mar Gil, Murat Yayla, Oluş Api

Statistics Editor
Resul Arısoy

Article info

Honokiol decreases intra-abdominal adhesion formation in a rat model. Perinatal Journal 2014;22(3):SE57 DOI: 10.2399/prn.14.S001084

Author(s) Information

Elif Ağaçayak1,
Senem Yaman Tunç1,
Mehmet Sait İçen1,
Ulaş Alabalık2,
Fatih Mehmet Fındık1,
Hatice Yüksel3,
Talip Gül1

  1. Dicle Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum Anabilim Dalı- Diyarbakır TR
  2. Dicle Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı- Diyarbakır TR
  3. Dicle Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı- Diyarbakır TR
Correspondence

Elif Ağaçayak, Dicle Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum Anabilim Dalı- Diyarbakır TR,

Publication History
Conflicts of Interest

No conflicts declared.

Objective
The purpose of this study was to investigate the effectiveness of honokiol, a natural molecule that was shown to have antioxidant effects, in prevention of intra-abdominal adhesion formation in a rat model.
Method 
This study was conducted on a total of 40 non-pregnant Sprague-Dawley rats which were divided into 4 groups as follows: sham, control, saline and honokiol groups. Both uterine horns of the rats in control, saline and honokiol groups were exposed and a 2 cm segment of the anti-mesenteric surface of both uterine horns was traumatized by a scalpel. The saline group was administered 2 ml of saline/day intraperitoneally for 5 days following the operation. The honokiol group, on the other hand, was administered honokiol intraperitoneally at a dose of 1 mg/kg/day for 5 days following the operation. On postoperative day 14, 3 mL of intracardiac blood sample was taken from the rats for biochemical analyses, and the rats were sacrificed this way. From the blood samples Total antioxidant status (TAC) and Total oxidant status (TOS) levels were studied. The adhesions were evaluated according to the microscopic adhesions classification of Zuhlke et al. Skin tissues were also evaluated for the presence of inflammation, granulation tissue and fibrosis.
Results 
Adhesion and inflammation scores were all significantly lower in the honokiol group compared to the saline and control groups (p<0.008)(Figure 1a,1b,1c).Similarly, fibrosis score was significantly lower in the honokiol group compared to the saline group (p<0.008)(Figure 2a,2b,2c). However, in comparison of TAS and TOS levels; there was no significant difference between groups (Table 1).
Conclusion 
Honokiol was found to be effective in prevention of intra-abdominal adhesion formation in a rat model. However, larger studies are needed to shed light on the exact role of honokiol in intra-abdominal adhesion formation and determine the molecular aspects of the promising results found in this study.
Keywords

Honokiol, intra-abdominal adhesions,rat

File/Dsecription
Figure 1
1.a. Grade 0 adhesion in a rat of the sham group (H&E, 100x) b. Grade 1 adhesion in a rat of the honokiol group (H&E, 100x) c. Grade 3 adhesion in a rat of the saline group (H&E, 100x) d. Grade 3 adhesion in a rat of the control group
Figure 2
2a. Normal appearance of the skin in a rat of the sham group (H&E, 100x) b. An area of complete epithelialization, worn off granulation tissue and decreased inflammation in a rat of the honokiol group (H&E, 100x) c. An area deprived of epithelializat
Table 1
Statistical findings (p values) of the study groups with regard to histological and biochemical data